期刊
SCIENCE OF THE TOTAL ENVIRONMENT
卷 648, 期 -, 页码 572-581出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.scitotenv.2018.08.119
关键词
Biosolid; Wastewater; Metagenomics; Methanogenic pathway; Microbiota
资金
- Colombian Administrative Department of Science, Technology and Innovation, COLCIENCIAS [111556933304 (CTO.535-2013)]
- Vicerrectoria de Investigacion, Universidad de Antioquia [CPT-1401]
- Spanish Ministry of Health (Instituto de Salud Carlos III)
- Ministerio de Economia y Competitividad-Fondo Europeo de Desarrollo Regional [CIBER 06/03, SAF2016-80532-R, PRX17/00500]
- Fundacio La Marato de TV3 [538/U/2016]
- University Jaume I [P1-1B2013-54, COGRUP/2016/06]
Abundance and diversity of microbial communities in biosolids are variable and poorly studied in the tropics, and it is known that rainfall is one of the events that could affect the phylogenetic and functional microbial structure. In the present study, using NGS technics, we studied the microbial diversity as well as the methanogenesis pathway in one of the largest WWTP in Colombia. Besides, we sampled and analyzed biosolids from rainy season and dry season. Phylogenetic classification showed a predominance of bacteria in both samples and difference in the dominant groups depending on the rainfall season. Whereas Pseudomonas was the dominant bacteria in the dry season, Coprothermobacter was in the rainy season. Archaea abundance was higher in the rainy season (11.5%) doubling dry season proportion. The bioreactor biogas production and total solids content showed similar results between rainy and dry season at the sampling dates. The most abundant Archaea related with methanogenesis was Methanosaeta, which is a methanogenic microorganism that exclusively uses acetate to produce methane. Moreover, annotation of the methanogenic pathway in the metagenome showed abundance in genes encoding AcetylCoA synthetases (ACSS), an enzyme that catalyzes acetate activation. Our results suggest that the microbial diversity was stable among the two time points tested, rainy season and dry season; and, although there were changes in the microbial abundance of dominant bacterial spedes, anaerobic digester performance is not affected. (C) 2018 Elsevier B.V. All rights reserved.
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