期刊
SCIENCE
卷 362, 期 6420, 页码 1306-+出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aau2599
关键词
-
资金
- Simons Foundation (SFLIFE) [288992]
- Chan Zuckerberg Biohub
- Sean N. Parker Center for Allergy and Asthma Research at Stanford University
- NSF Graduate Research Fellowship
- NSF Kou-I Yeh Stanford Graduate Fellowship
Immunoglobulin E (IgE) antibodies protect against helminth infections but can also cause life-threatening allergic reactions. Despite their role in human health, the cells that produce these antibodies are rarely observed and remain enigmatic. We isolated single IgE B cells from individuals with food allergies and used single-cell RNA sequencing to elucidate the gene expression and splicing patterns unique to these cells. We identified a surprising example of convergent evolution in which IgE antibodies underwent identical gene rearrangements in unrelated individuals. Through the acquisition of variable region mutations, these IgE antibodies gained high affinity and unexpected cross-reactivity to the clinically important peanut allergens Ara h 2 and Ara h 3. These findings provide insight into IgE B cell transcriptomics and enable biochemical dissection of this antibody class.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据