期刊
SCIENCE
卷 362, 期 6416, 页码 791-+出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aat9528
关键词
-
资金
- NIH [R01 CA103866, R01 CA129105, R37 AI47389]
- Department of Defense [W81XWH-07-0448]
- Cancer Research Institute Irvington Fellowship
- NATIONAL CANCER INSTITUTE [R01CA129105, R01CA103866] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R37AI047389] Funding Source: NIH RePORTER
One-carbon metabolism generates the one-carbon units required to synthesize many critical metabolites, including nucleotides. The pathway has cytosolic and mitochondrial branches, and a key step is the entry, through an unknown mechanism, of serine into mitochondria, where it is converted into glycine and formate. In a CRISPR-based genetic screen in human cells for genes of the mitochondrial pathway, we found sideroflexin 1 (SFXN1), a multipass inner mitochondrial membrane protein of unclear function. Like cells missing mitochondrial components of one-carbon metabolism, those null for SFXN1 are defective in glycine and purine synthesis. Cells lacking SFXN1 and one of its four homologs, SFXN3, have more severe defects, including being auxotrophic for glycine. Purified SFXN1 transports serine in vitro. Thus, SFXN1 functions as a mitochondrial serine transporter in one-carbon metabolism.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据