期刊
SCIENCE
卷 362, 期 6416, 页码 793-+出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aau1810
关键词
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资金
- Medical Research Council
- GABBA Ph.D. program (FCT fellowship) [PD/BD/52198/2013]
- Rosetrees Trust
- ARUK
- UK Dementia Research Institute [DRIImp17/18 Q3]
- Wellcome Senior Investigator award
- Alborada Trust of the ARUK Stem Cell Research Centre
- EPSRC [EP/J021199/1] Funding Source: UKRI
- MRC [MR/L023784/2, MR/L023784/1, UKDRI-5006, MC_U120088464] Funding Source: UKRI
Harnessing the potential of human stem cells for modeling the physiology and diseases of cortical circuitry requires monitoring cellular dynamics in vivo. We show that human induced pluripotent stem cell (iPSC)-derived cortical neurons transplanted into the adult mouse cortex consistently organized into large (up to similar to 100 mm(3)) vascularized neuronglia territories with complex cytoarchitecture. Longitudinal imaging of > 4000 grafted developing human neurons revealed that neuronal arbors refined via branch-specific retraction; human synaptic networks substantially restructured over 4 months, with balanced rates of synapse formation and elimination; and oscillatory population activity mirrored the patterns of fetal neural networks. Lastly, we found increased synaptic stability and reduced oscillations in transplants from two individuals with Down syndrome, demonstrating the potential of in vivo imaging in human tissue grafts for patient-specific modeling of cortical development, physiology, and pathogenesis.
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