期刊
SAINS MALAYSIANA
卷 47, 期 11, 页码 2617-2624出版社
UNIV KEBANGSAAN MALAYSIA
DOI: 10.17576/jsm-2018-4711-04
关键词
Colchicine; germplasm resources; pendimethalin; polyploid induction; tillered onion
资金
- Heilongjiang Province vegetable industry technology collaborative innovation system [HNWSCTX201701]
The shoot tip explants of the tillered onion line Z-018 were used as experimental materials. The effects of colchicine and pendimethalin on the induction of polyploid tillered onion were studied in vitro with different concentrations and treatment time. Treatment with 250 mu mol/L colchicine for 4 days caused a variant rate of 42.22% and a mortality rate of 36.67%, whereas treatment with 30 mu mol/L pendimethalin for 6 days caused a variant rate of 41.11% and a mortality rate of 24.44%. Subsequent cytological identification showed that tetraploid plants were successfully induced by both mutagens. Compared with the diploid plants, the tetraploid plants exhibited slower growth, thicker leaves, stems and roots, larger stomatal apparatus and increased number of chloroplasts. In addition, the obtained tetraploid plants have thicker tubular leaves, decreased number of tillers, larger bulbs and increased yield of the products. This provides the basis for future research on tillered onion germplasm resources.
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