期刊
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
卷 1851, 期 6, 页码 736-745出版社
ELSEVIER
DOI: 10.1016/j.bbalip.2014.12.008
关键词
Lipid binding domain; Rapamycin; Cryptochrome; Optogenetics; Optical manipulation; Phosphatidylinositol
资金
- NIDA NIH HHS [P30 DA018343] Funding Source: Medline
- NIDDK NIH HHS [R01 DK082700] Funding Source: Medline
- NINDS NIH HHS [R37 NS036251] Funding Source: Medline
Phosphoinositides (PIs) are minor components of cell membranes, but play key roles in cell function. Recent refinements in techniques for their detection, together with imaging methods to study their distribution and changes, have greatly facilitated the study of these lipids. Such methods have been complemented by the parallel development of techniques for the acute manipulation of their levels, which in turn allow bypassing the long-term adaptive changes implicit in genetic perturbations. Collectively, these advancements have helped elucidate the role of Pis in physiology and the impact of the dysfunction of their metabolism in disease. Combining methods for detection and manipulation enables the identification of specific roles played by each of the Pis and may eventually lead to the complete deconstruction of the PI signaling network. Here, we review current techniques used for the study and manipulation of cellular Pis and also discuss advantages and disadvantages associated with the various methods. This article is part of a Special Issue entitled Phosphoinositides. (C) 2014 Elsevier B.V. All rights reserved.
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