期刊
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY
卷 265, 期 -, 页码 141-152出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.resp.2018.10.008
关键词
Brainstem; Respiratory pattern generator; Inhibitory neurons; Glycine; Optogenetics
资金
- Deutsche Forschungsgemeinschaft [Hu797/7-1, Hu797/8-1]
- Alexander von Humboldt Foundation
Glycine is a primary inhibitory transmitter in the ventral medullary respiratory network, but the functional role of glycinergic neurons for breathing remains a matter of debate. We applied optogenetics to selectively modulate glycinergic neuron activity within regions of the rostral ventral respiratory column (VRC). Responses of the phrenic nerve activity to the light-driven stimulation were studied in the working heart-brainstem preparation from adult glycine transporter 2 Cre mice (GlyT2-Cre), which received a unilateral injection of a Cre-dependent AAV virus into Botzinger and preBotzinger Complex. Sustained light stimulation from the ventral medullary surface resulted in a substantial depression of the phrenic nerve (PN) frequency, which in most cases was compensated by an increase in PN amplitude. Periodic, burst stimulation with variable intervals could alter and reset respiratory rhythm. We conclude that unilateral activation of the rostral VRC glycinergic neurons can significantly affect respiratory pattern by lengthening the expiratory interval and modulating phase transition.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据