4.5 Article

Decidualization of Human Endometrial Stromal Fibroblasts is a Multiphasic Process Involving Distinct Transcriptional Programs

期刊

REPRODUCTIVE SCIENCES
卷 26, 期 3, 页码 323-336

出版社

SAGE PUBLICATIONS INC
DOI: 10.1177/1933719118802056

关键词

decidual stromal cells; endometrium; decidualization

资金

  1. NCI [U54CA209992]
  2. JTF [54860]
  3. National Cancer Institute of the National Institutes of Health [U54CA209992]
  4. European Commission Horizon 2020
  5. Marie Sklodowska-Curie IF [659668 EVOLPREG]
  6. Academy of Finland
  7. Jane and Aatos Erkko Foundation
  8. Paivikki and Sakari Sohlberg Foundation

向作者/读者索取更多资源

Decidual stromal cells differentiate from endometrial stromal fibroblasts (ESFs) under the influence of progesterone and cyclic adenosine monophosphate (cAMP) and are essential for implantation and the maintenance of pregnancy. They evolved in the stem lineage of placental (eutherian) mammals coincidental with the evolution of implantation. Here we use the well-established in vitro decidualization protocol to compare early (3 days) and late (8 days) gene transcription patterns in immortalized human ESF. We document extensive, dynamic changes in the early and late decidual cell transcriptomes. The data suggest the existence of an early signal transducer and activator of transcription (STAT) pathway dominated state and a later nuclear factor kappa B (NFKB) pathway regulated state. Transcription factor expression in both phases is characterized by putative or known progesterone receptor (PGR) target genes, suggesting that both phases are under progesterone control. Decidualization leads to proliferative quiescence, which is reversible by progesterone withdrawal after 3 days but to a lesser extent after 8 days of decidualization. In contrast, progesterone withdrawal induces cell death at comparable levels after short or long exposure to progestins and cAMP. We conclude that decidualization is characterized by a biphasic gene expression dynamic that likely corresponds to different phases in the establishment of the fetal-maternal interface.

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