期刊
RADIATION RESEARCH
卷 190, 期 6, 页码 634-644出版社
RADIATION RESEARCH SOC
DOI: 10.1667/RR15187.1
关键词
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资金
- Gordon and Betty Moore Foundation [4037, 4038]
We investigated the performance of several commonly used fluorescent dyes after exposure to a simulated Europa mission total ionizing radiation (lase of 300 krad (3 kGy) applied using a Co-60 source. Dyes irradiated in aqueous solution or as lyophilized powders were evaluated for absorbance and emission spectra, quantum yield, and where appropriate, ability to label cells or nucleic acids. Although some dyes showed significant increase or decrease in quantum yield with the dose, their spectra and cell-labeling properties remained essentially unchanged after irradiation in powder form. Irradiation in aqueous solution led to significantly greater changes, including a large blue shift in the DNA intercalator propidium iodide. These results suggest that many fluorescent probes are appropriate for use in astrobiological missions to Europa, but that SYTO9 and propidium iodide should be used with caution or not mixed with each other, as is commonly done in Live/Dead labeling applications. (C) 2018 by Radiation Research Society
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