4.1 Article

Key factors identified by proteomic analysis in maize (Zea mays L.) seedlings' response to long-term exposure to different phosphate levels

期刊

PROTEOME SCIENCE
卷 16, 期 -, 页码 -

出版社

BMC
DOI: 10.1186/s12953-018-0147-3

关键词

Proteomics; Maize seedlings; Low-pi tolerance; Pi transporter

资金

  1. Natural Science Foundation of Shandong Province [ZR2017BC021]
  2. Special Fund for Agro-scientific Research in the Public Interest [201503130]
  3. National Natural Science Foundation of China [31301271]
  4. major agricultural innovation project of Shandong Province [2017cxgx0311]

向作者/读者索取更多资源

Background: Maize seedlings are constantly exposed to inorganic phosphate (Pi)-limited environments. To understand how maize cope with low Pi (LP) and high Pi (HP) conditions, physiological and global proteomic analysis of QXN233 genotype were performed under the long-term Pi starvation and supplementation. Methods: We investigated the physiological response of QXN233 genotype to LP and HP conditions and detected the changes in ion fluxes by non-invasive micro-test technology and gene expression by quantitative real-time polymerase chain reaction. QXN233 was further assessed using vermiculite assay, and then proteins were isolated and identified by nano-liquid chromatography-mass spectrometry. Results: A negative relationship was observed between Na+ and Pi, and Na+ efflux was enhanced under HP condition. Furthermore, a total of 681 and 1374 were identified in the leaves and roots, respectively, which were mostly involved in metabolism, ion transport, and stress response. Importantly, several key Pi transporters were identified for breeding potential. Several ion transporters demonstrated an elaborate interplay between Pi and other ions, together contributing to the growth of QXN233 seedlings. Conclusion: The results from this study provide insights into the response of maize seedlings to long-term Pi exposure.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.1
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据