期刊
PROCESS BIOCHEMISTRY
卷 79, 期 -, 页码 185-194出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2018.12.006
关键词
pH-responsive copolymer; Pilot scale; Recycling aqueous two-phase systems; Partition coefficient; Antibiotics
资金
- National Natural Science Foundation of China [21376078]
Recycling pH-responsive aqueous two-phase system (pH-ATPS) has the advantages of low interfacial tension, high recovery, mild condition and low cost. In this paper, two pH-responsive copolymers 4% (w/v) P-ADB4.05 and 3% (w/v) P-MDB3.36 were used to construct a recycling pH-ATPS (the recoveries of two copolymers were both higher than 97%) for partition of demeclocycline and Lincomycin. They were successfully synthesized in pilot scale (500 L) through optimizing the monomer molar ratio and synthesis conditions and the yield of P-ADB4.05 and P-MDB3.36 were 97% and 96% respectively. The characteristics of two scaled-up copolymers were consistent with those synthesized in laboratory scale. The optimal partition coefficient (K) of demeclocycline were 4.223 with 20 mM LiCl at room temperature, respectively. For lincomycin, the K reached 2.629 in the presence of 4 mM MnCl2 at 45 degrees C. Partition of antibiotics in this pH-ATPS can be improved by varying pH, type and concentration of salts and temperature, which means it is available to antibiotics extraction and the further industrial separation.
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