期刊
POULTRY SCIENCE
卷 98, 期 4, 页码 1805-1819出版社
OXFORD UNIV PRESS
DOI: 10.3382/ps/pey499
关键词
broilers; thermal manipulation; interleukin-6 (IL-6); acute heat stress; immunity
资金
- Deanship of Research/Jordan University of Science Technology [128/2017]
In response to heat stress, interleukin-6 (IL-6) expression is upregulated in broiler chickens. The main aim of the present study was to evaluate the cumulative effects of thermal manipulation (TM) and subsequent acute heat stress (AHS) on the mRNA expression of IL-6 and genes involved in its induction pathways. The studied genes include IL-6, IL-1 beta, TNF-alpha, TLR2, TLR4, NF kappa B50, NF kappa B65, Hsp70, and HSF3 in the spleen and liver tissues. TM was carried out at 39 degrees C for 18 h and 65% relative humidity during days 10 to 18 of embryonic development, while AHS was stimulated by raising the temperature to 40 degrees C for 7 h on post-hatch day 28. During AHS at 0, 1, 3, 5, and 7 h, the spleen and liver were collected from all groups to measure the mRNA expression by relative-quantitation real-time RT-PCR, and the blood was collected to measure plasma IL-6 level. TM significantly reduced T-c during AHS for both breeds from 1 to 7 h time intervals. TM resulted in enhanced basal mRNA expression of IL-6, HSF3, and Hsp70, but decreased the basal mRNA level of TLR4. During heat stress, TM enhanced the expression dynamics of Hsp70, HSF3, IL-6, IL-1 beta, TNF-alpha, TLR2, TLR4, NF kappa B50, and NF kappa B65. The results of the current study indicate that TM enhanced the heat tolerance through improving the protective immunological response to heat stress by enhancing the expression of IL-6 and modulating the expression of genes important in its induction pathways.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据