期刊
PLANT JOURNAL
卷 97, 期 6, 页码 1089-1104出版社
WILEY
DOI: 10.1111/tpj.14181
关键词
QTL; salinity stress; seed germination; HAK transporter; Oryza sativa
资金
- National Natural Science Foundation of China [31771757, 31601387]
- National Key Research and Development Plan [2018YFD0100901]
- Science and Technology Project of Jiangsu Province [BE2016380]
- Natural Science Foundation of Jiangsu Province [BK20161451]
Seed germination is a complex trait determined by both quantitative trait loci (QTLs) and environmental factors and also their interactions. In this study, we mapped one major QTL qSE3 for seed germination and seedling establishment under salinity stress in rice. To understand the molecular basis of this QTL, we isolated qSE3 by map-based cloning and found that it encodes a K+ transporter gene, OsHAK21. The expression of qSE3 was significantly upregulated by salinity stress in germinating seeds. Physiological analysis suggested that qSE3 significantly increased K+ and Na+ uptake in germinating seeds under salinity stress, resulting in increased abscisic acid (ABA) biosynthesis and activated ABA signaling responses. Furthermore, qSE3 significantly decreased the H2O2 level in germinating seeds under salinity stress. All of these seed physiological changes modulated by qSE3 might contribute to seed germination and seedling establishment under salinity stress. Based on analysis of single-nucleotide polymorphism data of rice accessions, we identified a HAP3 haplotype of qSE3 that was positively correlated with seed germination under salinity stress. This study provides important insights into the roles of qSE3 in seed germination and seedling establishment under salinity stress and facilitates the practical use of qSE3 in rice breeding.
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