期刊
PLANT CELL AND ENVIRONMENT
卷 42, 期 5, 页码 1513-1531出版社
WILEY
DOI: 10.1111/pce.13508
关键词
GWAS; legume; Medicago truncatula; proline; salinity; SNP; vesicle trafficking
资金
- Samuel Roberts Noble Foundation
Salinity stress is an important cause of crop yield loss in many parts of the world. Here, we performed genome-wide association studies of salinity-stress responsive traits in 132 HapMap genotypes of the model legume Medicago truncatula. Plants grown in soil were subjected to a step-wise increase in NaCl concentration, from 0 through 0.5% and 1.0% to 1.5%, and the following traits were measured: vigor, shoot biomass, shoot water content, leaf chlorophyll content, leaf size, and leaf and root concentrations of proline and major ions (Na+, Cl-, K+, Ca2+, etc.). Genome-wide association studies were carried out using 2.5 million single nucleotide polymorphisms, and 12 genomic regions associated with at least four traits each were identified. Transcript-level analysis of the top eight candidate genes in five extreme genotypes revealed association between salinity tolerance and transcript-level changes for seven of the genes, encoding a vacuolar H+-ATPase, two transcription factors, two proteins involved in vesicle trafficking, one peroxidase, and a protein of unknown function. Earlier functional studies on putative orthologues of two of the top eight genes (a vacuolar H+-ATPase and a peroxidase) demonstrated their involvement in plant salinity tolerance.
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