期刊
PLANT CELL AND ENVIRONMENT
卷 42, 期 4, 页码 1287-1301出版社
WILEY
DOI: 10.1111/pce.13473
关键词
photosynthesis; chaperone; protein folding; RuBP carboxylation
资金
- Australian Research Council [LE0882289]
- Australian Research Council Centre of Excellence for Translational Photosynthesis [CE140100015]
- Bill and Melinda Gates Foundation [OPP1060461]
- Australian Research Council [LE0882289] Funding Source: Australian Research Council
The folding and assembly of Rubisco large and small subunits into L8S8 holoenzyme in chloroplasts involves many auxiliary factors, including the chaperone BSD2. Here we identify apparent intermediary Rubisco-BSD2 assembly complexes in the model C-3 plant tobacco. We show BSD2 and Rubisco content decrease in tandem with leaf age with approximately half of the BSD2 in young leaves (similar to 70 nmol BSD2 protomer.m(2)) stably integrated in putative intermediary Rubisco complexes that account for <0.2% of the L8S8 pool. RNAi-silencing BSD2 production in transplastomic tobacco producing bacterial L-2 Rubisco had no effect on leaf photosynthesis, cell ultrastructure, or plant growth. Genetic crossing the same RNAi-bsd2 alleles into wild-type tobacco however impaired L8S8 Rubisco production and plant growth, indicating the only critical function of BSD2 is in Rubisco biogenesis. Agrobacterium mediated transient expression of tobacco, Arabidopsis, or maize BSD2 reinstated Rubisco biogenesis in BSD2-silenced tobacco. Overexpressing BSD2 in tobacco chloroplasts however did not alter Rubisco content, activation status, leaf photosynthesis rate, or plant growth in the field or in the glasshouse at 20 degrees C or 35 degrees C. Our findings indicate BSD2 functions exclusively in Rubisco biogenesis, can efficiently facilitate heterologous plant Rubisco assembly, and is produced in amounts nonlimiting to tobacco growth.
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