期刊
PLANT AND CELL PHYSIOLOGY
卷 60, 期 4, 页码 826-834出版社
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcy248
关键词
Arabidopsis; Callus formation; Epigenetic modification; Histone methylation; Plant regeneration
资金
- Basic Science Research program by the National Research Foundation of Korea [NRF-2016R1D1A1B03931139]
- Basic Research Laboratory program by the National Research Foundation of Korea [NRF-2017R1A4A1015620]
- Next-Generation BioGreen 21 Program by the Rural Development Administration [PJ01319304]
Plant cells have a remarkable plasticity that allows cellular reprogramming from differentiated cells and subsequent tissue regeneration. Callus formation occurs from pericycle-like cells through a lateral root developmental pathway, and even aerial parts can also undergo the cell fate transition. Pluripotent calli are then subjected primarily to shoot regeneration in in vitro tissue culture. Successful completion of plant regeneration from aerial explants thus entails a two-step conversion of tissue identity. Here we show that a single chromatin modifier, ARABIDOPSIS TRITHORAX 4 (ATX4)/SET DOMAIN GROUP 16, is dynamically regulated during plant regeneration to address proper callus formation and shoot regeneration. The ATX4 protein massively activates shoot identity genes by conferring H3K4me3 deposition at the loci. ATX4-deficient mutants display strong silencing of shoot identity and thus enhanced callus formation. Subsequently, de novo shoot organogenesis from calli is impaired in atx4 mutants. These results indicate that a series of epigenetic reprogramming of tissue identity underlies plant regeneration, and molecular components defining tissue identity can be used as invaluable genetic sources for improving crop transformation efficiency.
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