期刊
PHYTOCHEMISTRY LETTERS
卷 30, 期 -, 页码 224-230出版社
ELSEVIER
DOI: 10.1016/j.phytol.2018.12.002
关键词
Rutaceae; High-performance countercurrent chromatography; Programmed cell death; Acridone alkaloids; Sarcodifurine C
资金
- Medical University of Lublin, Poland [DS28]
- Swiss National Science Foundation [316030_164095]
- Swiss National Science Foundation (SNF) [316030_164095] Funding Source: Swiss National Science Foundation (SNF)
This study was aimed at improving the current knowledge about the new Caledonian plant species Medicosma leratii, by developing efficient methods of extraction and isolation of specialized metabolites. Ultrasound-assisted extraction (UAE) significantly gave higher yields of the furoquinoline alkaloid skimmianine (22.34 mg/100 g) than accelerated solvent extraction (ASE, 1.37-12.36 mg/100 g). High-performance countercurrent chromatographic (HPCCC) separation of the methanol leaf extract with n-hexane-ethyl acetate-methanol-water (6:5:6:5 v/v) afforded skimmianine (1, 7.55 mg, HPLC-DAD purity 99.4%), normelicopidine (3, 1.87 mg, 95.6%), normelicopicine (4, 2.63 mg, 97.3%) and a new furoquinoline alkaloid named sarcodifurine C (2, 1.20 mg, 95.8%). The effect of the crude extract and its main constituent, skimmianine, on apoptosis, autophagy, and necrosis induction in tumor cells has also been investigated. The crude extract and skimmianine were found to be effective apoptosis inducers in HeLa cervical cancer cell line, but not in colon cancer SW620 and HT-29 cells. Additionally, skimmianine effectively potentiated the pro-apoptotic properties of the antincancer agent sorafenib in cervical cancer cells.
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