期刊
PHYTOCHEMISTRY
卷 156, 期 -, 页码 142-150出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.phytochem.2018.09.011
关键词
Cladonia rangiferina; Cladoniaceae; Algal gene expression; Apothecia; Atranorin; Fumarprotocetraric acid; Fungal gene expression; Laser microdissection; Thallus tissue
资金
- Department of Missions (Ministry of Higher Education and Scientific Research, Egypt)
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- NSERC-Vanier
- NSERC CGS-M
- NSERC [RGPAS 492880, RGPAS 478114]
The biosynthesis of two polyketides, atranorin and fumarprotocetraric acid, produced from a lichen-forming fungus, Cladonia rangiferina (L.) F. H. Wigg. was correlated with the expression of eight fungal genes (CrPKS1, CrPKS3, CrPKS16, Catalase (CAT), Sugar Transporter (MFsug), Dioxygenase (YQE1), C2H2 Transcription factor (C2H2), Transcription Factor PacC (PacC), which are thought to be involved in polyketide biosynthesis, and one algal gene, NAD-dependent deacetylase sirtuin 2 (AsNAD)), using laser microdissection (LMD). The differential gene expression levels within the thallus tissue layers demonstrate that the most active region for potential polyketide biosynthesis within the lichen is the outer apical region proximal to the photobiont but some expression also occurs in reproductive tissue. This is the first study using laser microdissection to explore gene expression of these nine genes and their location of expression; it provides a proof-of-concept for future experiments exploring tissue-specific gene expression within lichens; and it highlights the utility of LMD for use in lichen systems.
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