期刊
ACTA BIOMATERIALIA
卷 35, 期 -, 页码 293-304出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2016.02.002
关键词
CuInZnxS2+x quantum dots; LTVSPWY peptide; Cancer diagnosis; Breast cancer; HER2 targeting; Fluorescence imaging
资金
- National Centre for Research and Development under Research Grant Nanomaterials and their application to biomedicine [PBS1/A9/13/2012]
- International PhD Projects Program of Foundation for Polish Science
In this paper, the synthesis of alloyed CuInZnxS2+x quantum dots (ZCIS QDs), their transfer into aqueous solution via a polymer coating technique, and the use of these nanocrystals to selectively target HER2-positive cells, are reported. By optimizing first the ZnS shell deposition process onto the CuInS2 core, and next the encapsulation of the dots with the amphiphilic poly(maleic anhydride-alt-1-octadecene) (PMAO) polymer, water-dispersible ZCIS QDs were successfully prepared. The nanocrystals with a photoluminescence quantum yield of 35% were purified via centrifugation and ultracentrifugation and high quality nanoparticles with narrow size distributions and surface charges were obtained. After verifying the biocompatibility of PMO-coated ZCIS QDs, we coupled these nanocrystals with the LTVSPWY peptide and demonstrated via MTT assay that both bare and the peptide-linked QDs exhibit low cytotoxicity. The HER2-mediated delivery of the peptide-linked QDs was confirmed by confocal microscopy. This study indicates that as engineered QDs can efficiently be used as fluorescent nanoprobes for selective labelling of HER2-positive SKBR3 cancer cells. (C) 2016 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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