期刊
OPTICS LETTERS
卷 44, 期 2, 页码 391-394出版社
OPTICAL SOC AMER
DOI: 10.1364/OL.44.000391
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资金
- Research Grants Council, University Grants Committee (RGC, UGC) [CityU T42-103/16-N, EHKU701/17, HKU 17205215, HKU 17209018, HKU C7047-16G]
- National Natural Science Foundation of China (NSFC) [N_HKU712/16]
We demonstrate a volumetric two-photon microscopy (TPM) using the non-diffracting Airy beam as illumination. Direct mapping of the imaging trajectory shows that the Airy beam extends the axial imaging range around six times longer than a traditional Gaussian beam does along the propagation direction, while maintaining a comparable lateral width. Benefiting from its non-diffracting nature, the TPM with Airy beam illumination is able not only to capture a volumetric image within a single frame, but also to acquire image structures behind a strongly scattered medium. The volumetric specimen is mapped layer by layer under Gaussian mode, while the three-dimensional structure is projected to a single two-dimensional image under Airy mode, leading to a significantly increased acquisition speed. The performance of the TPM is evaluated employing a phantom of agarose gel imbedding fluorescent beads as well as a mouse brain slice. Finally, we showcase the penetration ability of the developed Airy TPM by imaging through a scattering environment. (C) 2019 Optical Society of America
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