期刊
OPTICS LETTERS
卷 43, 期 21, 页码 5484-5487出版社
OPTICAL SOC AMER
DOI: 10.1364/OL.43.005484
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资金
- Engineering and Physical Sciences Research Council (EPSRC) [EP/P030017/1, EP/R004854/1]
- European Union's Horizon 2020 Framework Programme (H2020) (BE-OPTICAL) [675512]
- EPSRC [EP/R004854/1, EP/P030017/1] Funding Source: UKRI
We present the first demonstration of three-photon excitation light-sheet fluorescence microscopy. Light-sheet fluorescence microscopy in single- and two-photon modes has emerged as a powerful wide-field, low-photodamage technique for Fast volumetric imaging of biological samples. We extend this imaging modality to the three-photon regime, enhancing its penetration depth. Our present study uses a conventional femtosecond pulsed laser at 1000 nm wavelength for the imaging of 450 mu m diameter cellular spheroids. In addition, we show, experimentally and through numerical simulations, the potential advantages in three-photon light-sheet microscopy of using propagation-invariant Bessel beams in preference to Gaussian beams. (C) 2018 Optical Society of America
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