期刊
ACTA ASTRONAUTICA
卷 129, 期 -, 页码 186-192出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.actaastro.2016.09.017
关键词
Simulated microgravity; Random position machine (RPM); Gene expression profile; Osteocyte; ATP-binding; Cytoskeleton
资金
- Natural Science Foundation of China [31570940, 31400725]
- Natural Science Basic Research Plan in Shaanxi Province of China [2015JM3078, 2015JQ3076]
- Fundamental Research Funds for the Central Universities [lzujbky-2015-188]
- New Century Excellent Talents in University [NCET-12-0469]
Bone undergoes dynamic modelling and remodelling processes, and it requires gravity-mediated mechanical stimulation for the maintenance of mineral content and structure. Osteocytes are the most commonly found cells in the mature bone, and they are sensitive to mechanical changes. The purpose of this study was to investigate the effects of microgravity simulated with a random position machine (RPM) on the gene expression profile of osteocytes. Genes sensitive to RPM treatment were sorted on the basis of biological processes, interactions and signalling pathways. Overall, 504 differentially expressed genes (DEGs) in osteocytes cultured under RPM conditions were found. The DEGs were further analysed using bioinformatics tools such as DAVID and iReport. A total of 15 ATP-binding and cytoskeleton-related genes were further confirmed by quantitative real-time PCR (qRT-PCR). Our findings demonstrate that the RPM affected the expression of genes involved in cytoskeleton remodelling and the energy-transfer process in osteocytes. The identification of mechanosensitive genes may enhance our understanding of the roles of osteocytes in mechanosensation and may provide some potential targets for preventing and treating bone-related diseases.
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