期刊
ACS NANO
卷 10, 期 6, 页码 5959-5970出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.6b01326
关键词
CXCR4; nanocluster; breast cancer; lung metastasis; positron emission tomography
类别
资金
- Mallinckrodt Institute of Radiology, Washington University
- Welch Foundation [A-0001]
- National Science Foundation [ECS-0335765]
As an emerging class of nanomaterial, nanoclusters hold great potential for biomedical applications due to their unique sizes and related properties. Herein, we prepared a Cu-64 doped gold nanocluster ((CuAuNC)-Cu-64, hydrodynamic size: 4.2 +/- 0.5 nm) functionalized with AMD3100 (or Plerixafor) for targeted positron emission tomography (PET) imaging of CXCR4, an up-regulated receptor on primary tumor and lung metastasis in a mouse 4T1 orthotopic breast cancer model. The preparation of targeted (CuAuNCs)-Cu-64-AMD3100 (4.5 +/- 0.4 nm) was done via one-step reaction with controlled conjugation of AMD3100 and specific activity, as well as improved colloid stability. In vivo pharmacokinetic evaluation showed favorable organ distribution and significant renal and fecal clearance within 48 h post injection. The expression of CXCR4 in tumors and metastasis was characterized by immunohistochemistry, Western blot, and reverse transcription polymerase chain reaction analysis. PET imaging with (CuAuNCs)-Cu-64-AMD3100 demonstrated sensitive and accurate detection of CXCR4 in engineered tumors expressing various levels of the receptor, while competitive receptor blocking studies confirmed targeting specificity of the nanoclusters. In contrast to nontargeted (CuAuNCs)-Cu-64 and Cu-64-AMD3100 alone, the targeted (CuAuNCs)-Cu-64-AMD3100 detected up-regulated CXCR4 in early stage tumors and premetastatic niche of lung earlier and with greater sensitivity. Taken together, we believe that (CuAuNCs)-Cu-64-AMD3100 could serve as a useful platform for early and accurate detection of breast cancer and metastasis providing an essential tool to guide the treatment. KEYWORDS: CXCR4, nanocluster, breast cancer, lung metastasis, positron emission tomography
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