期刊
ACS NANO
卷 11, 期 1, 页码 358-367出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.6b05949
关键词
organic nanoparticles; photoacoustic imaging; reaction-based probes; sulfenic acids
类别
资金
- Nanyang Technological University [M4081627.120, M4081533.080]
- Academic Research Fund Tier 1 from Singapore Ministry of Education [RG133/15: M4011559]
- Academic Research Fund Tier 2 from Ministry of Education in Singapore [MOE2016-T2-1-098]
Protein sulfenic acids play a key role in oxidative signal transduction of many biological and pathological processes; however, current chemical tools rely on visible fluorescence signals, limiting their utility to in vitro assays. We herein report reaction-based semiconducting polymer nanoprobes (rSPNs) with near-infrared absorption for in vivo photoacoustic (PA) imaging of protein sulfenic acids. rSPNs comprise an optically active semiconducting polymer as the core shielded with inert silica and poly(ethylene glycol) corona. The sulfenic acid reactive group (1,3-cyclohexanedione) is efficiently conjugated to the surface of nanoparticles via click chemistry. Such a nanostructure enables the specific recognition reaction between rSPNs and protein sulfenic acids without compromising the fluorescence and PA properties. In addition to in vitro tracking of the production of protein sulfenic acids in cancer cells under oxidative stress, rSPNs permit real-time PA and fluorescence imaging of protein sulfenic acids in tumors of living mice. This study thus not only demonstrates the first reaction-based PA probes with submolecular level recognition ability but also highlights the opportunities provided by hybrid nanoparticles for advanced molecular imaging.
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