期刊
ACS NANO
卷 10, 期 1, 页码 1189-1200出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.5b06501
关键词
zwitterion; GM3; Siglec1; stealth nanoparticle; hyperspectral imaging; antigen-presenting cells; gangliosides
类别
资金
- National Institutes of Health (NCI) [5R01CA138509]
- NATIONAL CANCER INSTITUTE [R01CA138509] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R01AI064099] Funding Source: NIH RePORTER
Membrane-wrapped nanoparticles represent a versatile platform for utilizing specific lipid receptor interactions, such as siallyllactose-mediated binding of the ganglioside GM3 to Siglecl (CD169), for targeting purposes. The membrane wrap around the nanoparticles not only serves as a matrix to incorporate GM3 as targeting moiety for antigen-presenting cells but also offers unique opportunities for constructing a biomimetic surface from lipids with potentially protein-repellent properties. We characterize nonspecific protein adsorption (corona formation) to membrane-wrapped nanoparticles with core diameters of approximately 35 and 80 nm and its effect on the GM3-mediated targeting efficacy as a function of surface charge through combined in vitro and in vivo studies. The stability and fate of the membrane wrap around the nanoparticles in a simulated biological fluid and after uptake in CD169-expressing antigen-presenting cells is experimentally tested. Finally, we demonstrate in hock immunization studies in mice that GM3-decorated membrane-wrapped nanoparticles achieve a selective enrichment in the peripheral regions of popliteal lymph nodes that contain high concentrations of CD169-expressing antigen-presenting cells.
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