期刊
ACS NANO
卷 10, 期 1, 页码 1602-1618出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.5b07253
关键词
multifunctional nanoparticles; multimodal imaging synthetic vaccines; toll-like receptors; drug delivery; immunotherapy; theranostics
类别
资金
- Spanish Ministry of Economy and Competitiveness [PRI-PIBIN-2011-0812, CTQ2014-54761-R, MAT2013-48169-R]
- Department of Industry of the Basque Country (ETORTEK)
- Diputacion de Guipuzcoa [Exp 63/15]
- Department of Education, Language Policy and Culture of the Basque Government
Development of vaccines to prevent and treat emerging new pathogens and re-emerging infections and cancer remains a major challenge. An attractive approach is to build the vaccine upon a biocompatible NP that simultaneously acts as accurate delivery vehicle and radiotracer for PET/SPECT imaging for ultrasensitive and quantitative in vivo imaging of NP delivery to target tissues/organs. Success in developing these nanovaccines will depend in part on having a correct NP size and accommodating and suitably displaying antigen and/or adjuvants (e.g., TLR agonists). Here we develop and evaluate a NP vaccine based on iron oxide-selective radio-gallium labeling suitable for SPECT(Ga-67)/PET(Ga-68) imaging 2 Targeted image-tracked nanovaccines and efficient delivery of antigen (OVA) and TLR 9 agonists (CpGs) using lipid-coated magnetite micelles. OVA, CpGs and rhodamine are easily accommodated in the hybrid micelles, and the average size of the construct can be controlled to be ca. 40 mu in diameter to target direct lymphatic delivery of the vaccine cargo to antigen presenting cells (APCs) in the lymph nodes (LNs). While the OVA/CpG-loaded construct showed effective delivery to endosomal TLR 9 in APCs, SPECT imaging demonstrated migration from the injection site to regional and nonregional LNs. In correlation with the imaging results, a range of in vitro and in vivo studies demonstrate that by using this microdosed nanosystem the cellular and humoral immune responses are greatly enhanced and provide protection against tumor challenge. These results suggest that these nanosystems have considerable potential for image-guided development of targeted vaccines that are more effective and limit toxicity.
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