期刊
NATURE METHODS
卷 16, 期 1, 页码 33-41出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41592-018-0219-4
关键词
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资金
- NIH [1R01NS102727, 1R01EB024261, 1R41MH112318, 1R01MH110932, 1RM1HG008525, 1DP1NS087724]
- Open Philanthropy Project, DARPA
- NSF [1734870]
- MIT Aging Brain Initiative/Ludwig Foundation
- IARPA [D16PC00008]
- US Army Research Laboratory
- US Army Research Office [W911NF1510548]
- US-Israel Binational Science Foundation [2014509]
- MIT Media Lab
- MIT Brain and Cognitive Sciences Department
- McGovern Institute
- Hertz Foundation Fellowship
- HHMI-Simons Faculty Scholars Program
- NATIONAL HUMAN GENOME RESEARCH INSTITUTE [RM1HG008525] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [R01EB024261] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF MENTAL HEALTH [R01MH110932, R41MH112318] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [R01NS102727, DP1NS087724] Funding Source: NIH RePORTER
Many biological investigations require 3D imaging of cells or tissues with nanoscale spatial resolution. We recently discovered that preserved biological specimens can be physically expanded in an isotropic fashion through a chemical process. Expansion microscopy (ExM) allows nanoscale imaging of biological specimens with conventional microscopes, decrowds biomolecules in support of signal amplification and multiplexed readout chemistries, and makes specimens transparent. We review the principles of how ExM works, advances in the technology made by our group and others, and its applications throughout biology and medicine.
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