期刊
NATURE METHODS
卷 15, 期 11, 页码 901-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41592-018-0180-2
关键词
-
资金
- Max Planck Society
- excellence cluster The Hamburg Centre for Ultrafast Imaging-Structure, Dynamics and Control of Matter at the Atomic Scale of the Deutsche Forschungsgemeinschaft
- People Programme (Marie Curie Actions) of the European Union's Seventh Framework Programme (FP7/2007-2013) under REA [623994]
- Alexander von Humboldt-Stiftung postdoctoral fellowship
- ETH Research Council [ETH-01 13-2]
- Natural Sciences and Engineering Research Council of Canada [RGPIN-2015- 04877]
- Canada Research Chairs program
- Burroughs Wellcome Fund for a Collaborative Research Travel Grant
We present a 'hit-and-return' (HARE) method for time-resolved serial synchrotron crystallography with time resolution from milliseconds to seconds or longer. Timing delays are set mechanically, using the regular pattern in fixed-target crystallography chips and a translation stage system. Optical pump-probe experiments to capture intermediate structures of fluoroacetate dehalogenase binding to its ligand demonstrated that data can be collected at short (30 ms), medium (752 ms) and long (2,052 ms) intervals.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据