期刊
NATURE CHEMICAL BIOLOGY
卷 15, 期 3, 页码 217-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41589-018-0186-1
关键词
-
资金
- National Science Foundation [MCB1615731, MCB1817675]
Predictable control over gene expression is essential to elicit desired synthetic cellular phenotypes. Although CRISPR-Cas9 offers a simple RNA-guided method for targeted transcriptional control, it lacks the ability to integrate endogenous cellular information for efficient signal processing. Here, we present a new class of riboregulators termed toehold-gated gRNA (thgRNA) by integrating toehold ribo-switches into sgRNA scaffolds, and demonstrate their programmability for multiplexed regulation in Escherichia coli with minimal cross-talks.
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