期刊
NATURE BIOTECHNOLOGY
卷 36, 期 12, 页码 1160-+出版社
NATURE PORTFOLIO
DOI: 10.1038/nbt.4273
关键词
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资金
- National Key Research and Development Program of China [2016YFD0101804]
- National Science Foundation of China [31788103, 31420103912]
- Chinese Academy of Sciences [QYZDY-SSW-SMC030, GJHZ1602]
- Thousand Talents Plan
Crop improvement by inbreeding often results in fitness penalties and loss of genetic diversity. We introduced desirable traits into four stress-tolerant wild-tomato accessions by using multiplex CRISPR-Cas9 editing of coding sequences, cis-regulatory regions or upstream open reading frames of genes associated with morphology, flower and fruit production, and ascorbic acid synthesis. Cas9-free progeny of edited plants had domesticated phenotypes yet retained parental disease resistance and salt tolerance.
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