期刊
NATURE BIOTECHNOLOGY
卷 36, 期 12, 页码 1156-1159出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nbt.4282
关键词
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资金
- NIH [R00AG040149, S10OD020072, R33CA225539]
- NSF CAREER Award [1653866]
- Welch Foundation [F1785]
- Robert J. Kleberg, Jr. and Helen C. Kleberg Foundation
- National Natural Science Foundation of China [81220108006]
- NSFC-NHMRC [81561128016]
- Thrust 2000 - Archie W. Straiton Endowed Graduate Fellowship in Engineering [1]
- Cockrell School of Engineering fellowship
- Thrust 2000 - Mario E. Ramirez Endowed Graduate Fellowship in Engineering
- NATIONAL CANCER INSTITUTE [R33CA225539] Funding Source: NIH RePORTER
We present tetramer-associated T-cell receptor sequencing (TetTCR-seq) to link T cell receptor (TCR) sequences to their cognate antigens in single cells at high throughput. Binding is determined using a library of DNA-barcoded antigen tetramers that is rapidly generated by in vitro transcription and translation. We applied TetTCR-seq to identify patterns in TCR cross-reactivity with cancer neoantigens and to rapidly isolate neoantigen-specific TCRs with no cross-reactivity to the wild-type antigen.
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