期刊
ACS NANO
卷 10, 期 1, 页码 1699-1710出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.5b07939
关键词
diagnostics; point-of-care; microfluidics; microarrays; single-enzyme analysis; ELISA; digital immunoassay
类别
资金
- Swiss National Science Foundation [CR23I2_140697]
- Swiss National Science Foundation (SNF) [CR23I2_140697] Funding Source: Swiss National Science Foundation (SNF)
Microfluidic diagnostic devices have the potential to transform the practice of medicine. We engineered a multiplexed digital-analog microfluidic platform for the rapid and highly sensitive detection of 3-4 biomarkers in quadruplicate in 16 independent and isolated microfluidic unit cells requiring only a single 5 mu L sample. We comprehensively characterized the platform by performing single enzyme and digital immunoassays, achieving single molecule detection and measured as low as similar to 10 fM (330 fg/mL) GFP in buffer and similar to 12 fM GFP in human serum. We applied our integrated digital detection mechanisni to multiplexed detection Of 1pM anti-Ebola IgG in human serum and were able to differentiate three common Ebola strains. To ascertain that the device can be applied in environments beyond clinical point-of-care settings, we developed a low-cost, portable hardware system to control and read out the microfluidic device and detected anti-Ebola IgG in ultralow volume whole blood samples to levels of 100 pM in a multiplexed assay format.
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