期刊
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
卷 845, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.mrgentox.2018.11.004
关键词
Nanoparticle; TiO(2)E171; Food additive; Toxicity; Intestine
资金
- Atomic Energy and Alternative Energies Commission (CEA) through the 'Toxicology' research program (IMAGINATOX grant)
- French Environment and Energy Management Agency (ADEME)
- French Agency for Food, Environmental and Occupational Health Safety (ANSES) [EST-2013/1/024]
- French National Research Agency (ANR) [ANR-16-CE34-0011-01]
- French Government's Investissements d'Avenir ANR program, through the A*MIDEX project [ANR-11-IDEX-0001-02, ANR-11-LABX-0064]
- hCOMET COST action [CA15132]
- Agence Nationale de la Recherche (ANR) [ANR-16-CE34-0011] Funding Source: Agence Nationale de la Recherche (ANR)
TiO2 particles are widely used in products for everyday consumption, such as cosmetics and food; their possible adverse effects on human health must therefore be investigated. The aim of this study was to document in vitro impact of the food additive E171, i.e. TiO2, and of TiO2 nanoparticles, on a co culture of Caco 2 and HT29-MTX cells, which is an in vitro model for human intestine. Cells were exposed to TiO2 particles three days after seeding, i.e. while they were not fully differentiated. Cell viability, reactive oxygen species (ROS) levels and DNA integrity were assessed, by MTT assay, DCFH-DA assay, alkaline and Fpg-modified comet assay and 8-oxo-dGuo measurement by HPLC-MS/MS. The mRNA expression of genes involved in ROS regulation, DNA repair via base-excision repair, and endoplasmic reticulum stress was assessed by RT-qPCR. Exposure to TiO2 particles resulted in increased intracellular ROS levels, but did not impair cell viability and did not cause any oxidative damage to DNA. Only minor changes in mRNA expression were detected. Altogether, this shows that E171 food additive and TiO2 nanoparticles only produce minor effects to this in vitro intestinal cell model.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据