期刊
ACS NANO
卷 10, 期 11, 页码 10382-10388出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.6b06235
关键词
silent amino acid residues; subunit interface redesign; geometry regulation; nanocage; ferritin reassembly
类别
资金
- National Natural Science Foundation of China [31271826, 31471693]
- Chinese Universities Scientific Fund for Graduate Students of the China Agricultural University [2015sp004]
- Grants-in-Aid for Scientific Research [16H04909] Funding Source: KAKEN
Rendering the geometry of protein-based assemblies controllable remains challenging. Protein shell-like nanocages represent particularly interesting targets for designed assembly. Here, we introduce an engineering strategy key subunit interface redesign (KSIR) that alters a natural subunit-subunit interface by selective deletion of a small number of silent amino acid residues (no participation in interfacial interactions) into one that triggers the generation of a non-native protein cage. We have applied KSIR to construct a non-native 48-mer nanocage from its native 24-mer recombinant human H-chain ferritin (rHuHF). This protein is a heteropolymer composed of equal numbers of two different subunits which are derived from one polypeptide. This strategy has allowed the study of conversion between protein nanocages with different geometries by re-engineering key subunit interfaces and the demonstration of the important role of the above-mentioned specific residues in providing geometric specificity for protein assembly.
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