4.6 Article

(-)-Epigallocatechin-3-Gallate Decreases Osteoclastogenesis via Modulation of RANKL and Osteoprotegrin

期刊

MOLECULES
卷 24, 期 1, 页码 -

出版社

MDPI
DOI: 10.3390/molecules24010156

关键词

catechin; EGCG; osteoclast; osteoclastogenesis; RANK; RANKL; OPG; RAW 264; 7 cells

资金

  1. National Health Research Institute of Taiwan [NHRI-EX101-9935EI]
  2. Kaohsiung Medical University Hospital [KMUH107-7R54]
  3. Kaohsiung Medical University [KMU-TP105B10, KMU-DK105009]
  4. National Science Council [NSC 94-2314-B-037-021-]
  5. [MOST 104-2314-B-037-032-MY3]

向作者/读者索取更多资源

Osteoporosis is the second most common epidemiologic disease in the aging population worldwide. Previous studies have found that frequent tea drinkers have higher bone mineral density and less hip fracture. We previously found that (-)-epigallocatechin gallate (EGCG) (20-100 mu mol/L) significantly suppressed receptor activator of nuclear factor-kB ligand (RANKL)-induced osteoclastogenesis and pit formation via inhibiting NF-B transcriptional activity and nuclear transport of NF-B in RAW 264.7 cells and murine primary bone marrow macrophage cells. The most important regulation in osteoclastogenesis is the receptor activator of nuclear factor-kB/RANKL/osteoprotegrin (RANK/RANKL/OPG) pathway. In this study, we used the coculture of RAW 264.7 cells and the feeder cells, ST2, to evaluate how EGCG regulated the RANK/RANKL/OPG pathway in RAW 264.7 cells and ST2 cells. We found EGCG decreased the RANKL/OPG ratio in both mRNA expression and secretory protein levels and eventually decreased osteoclastogenesis by TRAP (+) stain osteoclasts and TRAP activity at low concentrations1 and 10 mu mol/Lvia the RANK/RANKL/OPG pathway. The effective concentration can be easily achieved in daily tea consumption. Taken together, our results implicate that EGCG could be an important nutrient in modulating bone resorption.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.6
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据