期刊
MOLECULES
卷 23, 期 12, 页码 -出版社
MDPI
DOI: 10.3390/molecules23123222
关键词
UHPLC-LTQ-Orbitrap; iridoids; flavonoids; xanthones; characteristic fragmentation pathways; wine-processing; Gentiana radix
资金
- National Standardization Project of Traditional Chinese Medicine-Standardization Construction of Six Kinds of Chinese Herbal Medicines, such as Scutellaria [ZYBZH-Y-TJ-43]
- Liaoning Province's Innovative Talents Support Scheme [LR2016037]
Gentiana radix is used in traditional Chinese medicine and has functions of clearing heat and drying dampness, as well as purging liver and gallbladder fire. A highly sensitive and effective strategy for rapid screening and identification of target constituents has been developed by using ultra high-performance liquid chromatography coupled with linear ion trap-Orbitrap mass spectrometry (UHPLC-LTQ-Orbitrap) in crude and wine-processed Gentiana radix. Based on the accurate mass measurement (<5 ppm), retention times, and MS fragmentation ions, 52 constituents were unambiguously or tentatively characterized from Gentiana radix, including 21 iridoids, 11 flavonoids, 19 xanthones, and a triterpenoid. This study demonstrated that the established method could be a rapid, effective analytical tool for screening and characterization of compounds in the complex systems of Gentiana radix. By comparing the structure and peak areas of chemical constituents in crude and wine-processed Gentiana radix, we found that some compounds in crude and wine-processed Gentiana radix were significantly different.
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