期刊
MOLECULES
卷 23, 期 12, 页码 -出版社
MDPI
DOI: 10.3390/molecules23123350
关键词
nitroimidazole; honey; liquid chromatography; mass spectrometry; d-SPE
资金
- National Key R&D Program of China [2017YFC1601300]
- Sanming Project of Medicine in Shenzhen [SZSM201611068]
- National Natural Science Foundation of China [31772794]
An ultra-high performance liquid chromatography-tandem quadrupole mass spectrometry (UHPLC-MS/MS) method was developed and validated for confirmatory analysis of four nitroimidazoles and three hydroxy metabolites in honey. Honey samples were dissolved in 2% formic acid solution and nitroimidazoles and metabolites were isolated and enriched by dispersive-solid phase extraction using mixed-mode strong cation-exchange sorbent. The determination involves separation of analytes on an UHPLC C-18 column and detection by multiple reaction monitoring in positive ionization mode. The recovery of the method was ranged from 90.2 to 105.6% with inter-day relative standard deviations of less than 11.2%. The limits of detection and limits of quantification were in the ranges of 0.02-0.07 mu g/kg and 0.05-0.2 mu g/kg, respectively. Honey samples from the market were analyzed to demonstrate the applicability of the proposed method.
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