期刊
MOLECULAR CELL
卷 72, 期 4, 页码 700-+出版社
CELL PRESS
DOI: 10.1016/j.molcel.2018.09.013
关键词
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资金
- NIH R01 grants [GM37706, GM37949]
- Stanford Graduate Fellowship
- HHMI International Student Research Fellowship
- Intramural Program of the U.S. Department of Health and Human Services
- Spanish Ministerio de Economia, Industria y Competitividad'' - FEDER funds [BFU2017-85464]
- DOE Office of Science User Facility [DE-AC02-05CH11231]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM037949, R01GM037706] Funding Source: NIH RePORTER
Prokaryotic CRISPR-Cas systems provide adaptive immunity by integrating portions of foreign nucleic acids (spacers) into genomic CRISPR arrays. Cas6 proteins then process CRISPR array transcripts into spacer-derived RNAs (CRISPR RNAs; crRNAs) that target Cas nucleases to matching invaders. We find that a Marinomonas mediterranea fusion protein combines three enzymatic domains (Cas6, reverse transcriptase [RT], and Cas1), which function in both crRNA biogenesis and spacer acquisition from RNA and DNA. We report a crystal structure of this divergent Cas6, identify amino acids required for Cas6 activity, show that the Cas6 domain is required for RT activity and RNA spacer acquisition, and demonstrate that CRISPR-repeat binding to Cas6 regulates RT activity. Co-evolution of putative interacting surfaces suggests a specific structural interaction between the Cas6 and RT domains, and phylogenetic analysis reveals repeated, stable association of free-standing Cas6s with CRISPR RTs in multiple microbial lineages, indicating that a functional interaction between these proteins preceded evolution of the fusion.
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