期刊
MOLECULAR AND CELLULAR BIOCHEMISTRY
卷 454, 期 1-2, 页码 203-214出版社
SPRINGER
DOI: 10.1007/s11010-018-3464-z
关键词
Apoptosis; Bitter taste receptor; Epithelial ovarian cancer; Prostate cancer; Noscapine; G protein coupled receptor
类别
资金
- Natural Sciences and Engineering Research Council of Canada (NSERC) [RGPIN-355310-2013]
- Beatrice Hunter Cancer Research Institute (BHCRI)
- BHCRI
- CancerCare Manitoba Foundation
- Canadian Institutes of Health Research
- Motorcycle Ride for Dad-Nova Scotia Chapter
Bitter taste receptors (Tas2Rs) are a subfamily of G-protein coupled receptors expressed not only in the oral cavity but also in several extra-oral tissues and disease states. Several natural bitter compounds from plants, such as bitter melon extract and noscapine, have displayed anti-cancer effects against various cancer types. In this study, we examined the prevalence of Tas2R subtype expression in several epithelial ovarian or prostate cancer cell lines, and the functionality of Tas2R14 was determined. qPCR analysis of five TAS2Rs demonstrated that mRNA expression often varies greatly in cancer cells in comparison to normal tissue. Using receptor-specific siRNAs, we also demonstrated that noscapine stimulation of ovarian cancer cells increased apoptosis in ovarian cancer cells in a receptor-dependent, but ROS-independent manner. This study furthers our understanding of the function of Tas2Rs in ovarian cancer by demonstrating that their activation has an impact on cell survival.
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