期刊
ACS CHEMICAL BIOLOGY
卷 11, 期 8, 页码 2216-2221出版社
AMER CHEMICAL SOC
DOI: 10.1021/acschembio.6b00090
关键词
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资金
- JSPS [26410181]
- Grants-in-Aid for Scientific Research [26410181, 16K18846] Funding Source: KAKEN
We have developed oligodeoxynucleotides (ODNs) that modify primary amines to produce 5,6-dimethoxy 3-methyleneisoindolin-1-one. Compared to the oxygen isosteric fluorophore, 4,5-dimethoxyphthalimide, this methyleneisoindolinone was more stable and exhibited an 85 nm blue-shifted fluorescent emission (lambda(max) at 425 nm) with an intensity comparable to that of the phthalimide. Reaction of the DNA-binding domain of Escherichia coli DnaA protein with an ODN containing its binding sequence efficiently afforded a modified fluorescent protein at a specific lysine residue in the proximity of the ODN. A full-length DnaA protein was also successfully fluorescently labeled. These results demonstrate the potential utility of the ODNs developed in this study for the fluorescent labeling of DNA-interacting protein at the lysine residue of interest.
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