期刊
ACS APPLIED MATERIALS & INTERFACES
卷 8, 期 11, 页码 6841-6851出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.6b00004
关键词
TiO2; Bi2WO6; binanosheet; photocatalytic disinfection; leakage of cell contents
资金
- National Natural Science Foundation of China [21377061, 31170473]
- joint Guangdong project [U1133006]
- MOE Innovative Research Team in University [IRT 13024]
- Natural Science Foundation of Tianjin [15JCYBJC48400, 15JCZDJC41200]
TiO2Bi2WO6 binanosheet (TBWO), synthesized by a facile two-step hydrothermal method, was used as an effective visible-light-driven photocatalyst for the inactivation of E. coli and was characterized by TEM, SEM, XRD, FTIR, XPS, and BET. A series of TBWOs with different doping ratios of TiO2 loading from 10 to 55 wt % were synthesized. Among all of the TBWOs, 40% TBWO exhibited the best bacteria disinfection efficiency, and the quantity of viable bacteria could reach 10 degrees with 40% TBWO (100 mu g/mL) after being illuminated for 4 h. Furthermore, the confocal fluorescent-based cell live/dead test and the SEM technology were applied to verify the photocatalytically lethal effect toward E. coli and the rupture of bacterial membranes. The leak of bacterial contents, including the bacterial genome represented by relevant 16srDNA, and total protein were detected by PCR and bicinchoninic acid assay. In this work, the antibacterial mechanism was studied by employing photoelectrochemical techniques, electron spin resonance (ESR), and scavengers of different reactive species, revealing the pivotal roles of electron hole (h(+)) and electron (e(-)) in the photocatalytic process. In addition, the center dot O-2(-) and center dot OH radicals were also detected in the TBWOs system by ESR. It was found that the adsorption of visible light and separation of photogenerated carriers within TiO2 have been largely promoted after being coupled with Bi2WO6, which should be responsible for the improved bactericidal effect.
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