期刊
MICROCIRCULATION
卷 26, 期 1, 页码 -出版社
WILEY
DOI: 10.1111/micc.12515
关键词
bioenergetics; cell behavior; endothelial cells; exosomes; TNF-alpha
资金
- CAMS Initiative for Innovative Medicine (CAMS-I2M) [2016-I2M-3-006]
Objective To investigate the effects of TNF-alpha-induced exosomes release on the biological behavior, metabolism, and bioenergetics of HUVECs. Methods Exosomes were isolated from conditioned media of HUVECs by ultracentrifugation after treatment with or without TNF-alpha. HUVECs were treated with or without TNF-alpha, or different concentrations of exosomes isolated from conditioned media with or without TNF-alpha induction (T-Exo and C-Exo, respectively). Results The results showed that TNF-alpha significantly inhibited migration, tube formation, and increased apoptosis rate of HUVECs compared with controls. Furthermore, TNF-alpha-induced exosomes (T-Exo) rather than C-Exo, indicated similar effects to inhibit migration, tube formation and promote endothelial apoptosis. Although TNF-alpha treatment did not show a statistical difference, T-Exo significantly inhibited extracellular OCR compared with controls. T-Exo could significantly inhibited intracellular OCR in a hypoxia condition. TNF-alpha significantly increased L-ECA compared with control cells, and T-Exo showed similar stimulative effect on L-ECA. Conclusions TNF-alpha-induced exosomes could significantly (a) change migration, tube formation, and apoptosis; (b) inhibit endothelial extracellular OCR and intracellular OCR (hypoxia); (c) increase glycolysis rate of the endothelial cells. These data provide new evidence for exploring endothelial behavior regulation using exosomes and their effects on endothelial metabolism and bioenergetics.
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