期刊
MICROCHIMICA ACTA
卷 185, 期 12, 页码 -出版社
SPRINGER WIEN
DOI: 10.1007/s00604-018-3081-2
关键词
Electrochemical aptasensor; Mycobacterium tuberculosis; MPT64; UiO-66-NH2; Metal-organic framework; Horseradish peroxidase
资金
- National Natural Science Foundation of China [21675177]
- Science and Technology Planning Project of Guangdong Province [2016B030303002]
- Natural Science Foundation of Guangdong Province [2018A030310142]
- Medical Scientific Research Foundation of Guangdong Province [A2017033]
An ultrasensitive aptasensor is described for the voltammetric determination of the Mycobacterium tuberculosis antigen MPT64 in human serum. Firstly, an amino-modified Zr(IV) based metal-organic framework (MOF; type UiO-66-NH2;made up from Zr6O32 units and 2-amino-terephthalate linkers) with a high specific surface was synthesized and used as the carrier of the gold nanoparticles and the aptamers. Then the signalling nanoprobe was fabricated after the horseradish peroxidase was cast on the nanomaterials. The two aptamers with synergistic effect on binding MPT64 were anchored on the gold electrode. Differential pulse voltammetry indicated that the peak current is highest if the ratio of the two aptamers is 1:1. The assay has a wide linear response range (0.02 to 1000pg mL( -1) of MPT64) and a 10fg mL( -1) detection limit at a working potential ofaround -96mV (vs Ag/AgCl). The results show this biosensor to be a viable tool for detection of tuberculosis atan early stage.
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