期刊
MICROCHIMICA ACTA
卷 185, 期 11, 页码 -出版社
SPRINGER WIEN
DOI: 10.1007/s00604-018-3061-6
关键词
Ratiometric method; Fluorescence assay; Heparin; Polyethyleneimine; CdTe quantum dots; Carbon dots; Fluorescence quenching; Dually emitting probe
资金
- National Natural Science Foundation of China [21775100]
CdTe quantumdots (QDs) were integrated with polyethyleneimine-coated carbon dots (PEI-CDs) to form a dually emitting probe for heparin. The red fluorescence of the CdTe QDs is quenched by the PEI-CDs due to electrostatic interactions. In the presence of heparin, the blue fluorescence of PEI-CDs remains unaffected, while its quenching effect on the fluorescence of CdTe QDs is strongly reduced. A ratiometric fluorometric assay was worked out. The ratio of the fluorescences at 595 and 436 nm serves as the analytical signal. Response is linear in the concentration range of 50-600 ng center dot mL-1 (0.1-1.2 U center dot mL-1) of heparin. The limit of detection is 20 ng center dot mL-1 (0.04 U center dot mL-1). This makes the method a valuable tool for heparin monitoring during postoperative and long-term care. This assay is relatively free from the interference by other analogues which commonly co-exist with heparin in samples, and it is more robust than single-wavelength based assays.
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