期刊
MICROCHIMICA ACTA
卷 186, 期 2, 页码 -出版社
SPRINGER WIEN
DOI: 10.1007/s00604-018-3188-5
关键词
Kanamycin (KANA); Aptamer; Electrochemical detection; Signal-on Aptasensor; Chicken sample
资金
- National Natural Science Foundation of China [61301037]
- Henan Science and Technology Cooperation Project [172106000014]
- Cultivation Plan for Young Core Teachers in Universities of Henan Province [2017GGJS072]
- Youth Backbone Teacher Training Program of Henan University of Technology [21420004]
- Master's Degree Thesis Cultivation Project of Henan University of Technology
A signal-on aptasensor is described for the voltammetric determination of kanamycin (KANA). Au@Pt core-shell nanoparticles with large surface and good electrical conductivity were synthetized and act as both a conductive material and as the carrier for complementary strands (CS2) and thionine (TH). In the presence of KANA, the electrochemical response of TH changes due to hybridization between CS1 immobilized on the electrode and the Au@Pt-CS2/TH system. The peak current increases linearly with the logarithm of the KANA concentration in the range from 1 pM to 1M, and the limit of detection is 0.16 pM. The sensor was characterized in terms of selectivity, reproducibility and stability, and satisfactory results were obtained. It was also utilized for the determination of KANA in (spiked) chicken samples. The recoveries (95.8-103.2%) demonstrate the potential of the method for KANA detection in real samples.
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