期刊
MICROCHEMICAL JOURNAL
卷 144, 期 -, 页码 319-328出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.microc.2018.09.021
关键词
Multiple Reaction Monitoring (MRM); Proteomics; Ancient proteins; Cultural heritage; Paintings; LC-MSMS
资金
- POR, Parco Archeologico Urbano di Napoli (PAUN)
Identification of proteins in paintings and polychrome objects is a challenge, which requires the development of tailored analytical approaches. In the present study, a targeted proteomics approach was developed for discriminating among the three most common proteinaceous materials used as paint binders, i.e. milk, egg, and animal glue. In this study a specific database of peptides was created based on tandem MS analyses of tryptic digests of several paint samples collected from a variety of art objects of different ages and conservation conditions. Specific peptide markers of each protein were then selected and monitored by LC-MSMS in Multiple Reaction Monitoring (MRM) ion mode, together with their specific precursor ion-product ion transitions, as defined by their unique amino acid sequence. The developed method enabled a sensitive and reliable detection of the target peptides in a selection of case studies, leading to the unambiguous identification of the proteins used as paint binders. The method showed greatly increased sensitivity compared to currently available strategies.
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