期刊
MICROBIAL PATHOGENESIS
卷 128, 期 -, 页码 414-422出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.micpath.2018.12.047
关键词
Newcastle disease virus (NDV); Exosome; Protein A/G; Nucleoprotein (NP); Infection
资金
- National Natural Science Foundation of China [31772735, 31472195]
- National Key Research and Development Program of China [2018YFD0500100]
- Jiangsu Provincial Natural Science Foundation of China [BK20180299]
- Key Technology Research and Development Project of Jilin Province [20180201021NY]
Exosomes are micro messengers encapsulating RNA, DNA, and proteins for intercellular communication associated with various physiological and pathological reactions. Several viral infection processes have been reported to pertain to exosomal pathways. However, because of the difficulty in obtaining avian-sourced exosomes, avian virus-related exosomes are scarcely investigated. In this study, we developed a protein A/G-correlated method and successfully obtained the Newcastle disease virus-related exosome (NDV Ex). These exosomes promoted NDV propagation, proven by both GW4869-mediated deprivation and exosomal supplementation. Viral structural proteins NP and F were detected in the NDV Ex and further investigation indicated that the NP protein can be transferred to DF-1 cells through exosomes. The intracellular NP protein exhibited viral replication-promoting and cytokine-suppressing abilities. Therefore, NDV infection produces exosomes, which transfer viral NP protein and promote NDV infection, emphasizing the importance of exosomes in an NDV infection.
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