期刊
ACS APPLIED MATERIALS & INTERFACES
卷 8, 期 40, 页码 26578-26590出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.6b09074
关键词
nanotube; surface grafting; drug delivery; cell viability; tumor therapy
资金
- National High Technology Research and Development Program of China [2015AA020915]
- National Natural Science Foundation of China [51473069, 81473115, 51502113]
- Guangdong Natural Science Funds for Distinguished Young Scholar [52013050014606, S20120011316]
- Science and Technology Program of Guangzhou, China [2013J4100100, 201610010026]
- Guangdong Special support program [2014TQ01C127]
- Fundamental Research Funds for the Central Universities [21615204]
Halloysite nanotubes (HNTs) are natural aluminosilicates with unique hollow lumen structure, also having high specific area, good biocompatibility, nontoxicity, and low price. Here, we designed a chitosan oligosaccharide-grafted HNTs (HNTs-g-COS) as a doxorubicin (DOX) carrier for treating breast cancer both in vitro and in-vivo. The structure of HNTs-g-COS was first characterized by various methods. HNTs-g-COS showed positively charged surface and improved hemocompatibility. DOX-loaded HNTs-g-COS (DOX@HNTs-g-COS) released in cell lysate in a controlled manner. The IC50 value of DOX@HNT5-g-COS toward MCF-7 cells was 1.17 mu g mL(-1), while it was 2.43 mu g mL(-1) for free DOX. DOX@HNTs-g-COS increased the apoptosis effects of MCF-7 cells as shown in flow cytometry results. Also, reactive oxygen species of cells induced by DOX@HNTs-g-COS were drug-dose-dependent. DOX@HNTs-g-COS could enter the MCF-7 cells and induce mitochondrial damage as well as attack the nuclei. The in vivo antitumor effect of DOX@HNTs-g-COS was investigated in 4T1-bearing mice. The tumor-inhibition ratio of DOX@HNTs-g-COS was 83.5%, while it was 46.1% for free DOX. All mice treated with DOX@HNTs-g-COS survived over 60 days,. DOX@HNT5-g-COS showed fewer ruptured cardiomyocytes and no obvious systemic toxicity. Therefore, the rational designed HNTs nanocarrier for chemotherapy drug showed promising applications in tumor treatment.
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