期刊
ACS APPLIED MATERIALS & INTERFACES
卷 8, 期 7, 页码 4442-4455出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.5b12141
关键词
hydrogel; blank platform; ECM; thiol-ene click chemistry; 3D cell encapsulation
资金
- National Nature Science Foundation of China [31271016, 31370975, 51573127, 81101448]
- Natural Science Foundation of Hebei Province [H2012401017]
Three-dimensional (3D) cell encapsulation in hydrogel provides superb methods to investigate the biochemical cues in directing cellular fate and behaviors outside the organism, the primary step of which is to establish suitable blank platform to mimic and simplify native ECM microenvironment. In this study, zwitterionic starch-based clickable hydrogels were fabricated via a copper- and light-free Michael-type thiol-ene addition reaction between acylated-modified sulfobetaine-derived starch (SB-ST-A) and dithiol-functionalized poly(ethylene glycol) (PEG-SH). By incorporating antifouling SB-ST and PEG, the hydrogel system would be excellently protected from nontarget protein adsorption to act as a blank platform. The hydrogels could rapidly gel under physiological conditions in less than 7 min. Dynamic rheology experiments suggested the stiffness of the hydrogel was close to the native tissues, and the mechanical properties as well as the gelation times and swelling behaviors could be easily tuned by varying the precursor proportions. The protein and cell adhesion assays demonstrated that the hydrogel surface could effectively resist nonspecific protein and cell adhesion. The degradation study in vitro confirmed that the hydrogel was biodegradable. A549 cells encapsulated in the hydrogel maintained high viability (up to 93%) and started to proliferate in number and extend in morphology after 2 days' culture. These results indicated the hydrogel presented here could be a potential candidate as blank platform for 3D cell encapsulation and biochemical cues induced cellular behavior investigation in vitro.
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