4.7 Article

Misincorporation signatures for detecting modifications in mRNA: Not as simple as it sounds

期刊

METHODS
卷 156, 期 -, 页码 53-59

出版社

ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2018.10.011

关键词

RNA modification; Epitranscriptome; Next generation sequencing; Misincorporation signatures

向作者/读者索取更多资源

Post-transcriptional modification on mRNA has become a field of intense interest in recent years, and next generation sequencing based technologies are constantly emerging to detect an increasing number of modifications at a transcriptome-wide level. Some of these approaches are based on identification of misincorporation events induced by reverse transcriptase at modified sites. Although conceptually trivial, sensitive and specific identification of such events is a challenge prone to a surprising number of artifacts, which can lead to substantially inflated estimates of the abundance of diverse modifications. Here we discuss the sources of some of these artifacts and delineate approaches to overcome them.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据